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Evaluation of alternating tangential flow filtration <t> (ATF) </t> for continuous media <t> perfusion </t> and cell retention.
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Evaluation of alternating tangential flow filtration <t> (ATF) </t> for continuous media <t> perfusion </t> and cell retention.
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Evaluation of alternating tangential flow filtration <t> (ATF) </t> for continuous media <t> perfusion </t> and cell retention.
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Evaluation of alternating tangential flow filtration <t> (ATF) </t> for continuous media <t> perfusion </t> and cell retention.
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Evaluation of alternating tangential flow filtration <t> (ATF) </t> for continuous media <t> perfusion </t> and cell retention.
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Evaluation of alternating tangential flow filtration (ATF) for continuous media perfusion and cell retention.

Journal: Frontiers in Medical Technology

Article Title: Enabling Allogeneic T Cell-Based Therapies: Scalable Stirred-Tank Bioreactor Mediated Manufacturing

doi: 10.3389/fmedt.2022.850565

Figure Lengend Snippet: Evaluation of alternating tangential flow filtration (ATF) for continuous media perfusion and cell retention.

Article Snippet: As the pore size of the hollow fibers in Repligen's ATF is 0.2 mm, we have employed ATF as a cell retention device for continuous media perfusion in STR.

Techniques: Filtration, Concentration Assay

The T cell activation in STR with ATF mediated continuous perfusion results in T cells with stemness over terminal differentiation or exhaustion: (A) Prominent subsets of T cells before, during and after expansion; (B) Differentiation status of T cells before, during and after expansion; and (C) Senescence and exhaustion of T cells before, during and after expansion. The values represent average of 50,000 single cells per condition.

Journal: Frontiers in Medical Technology

Article Title: Enabling Allogeneic T Cell-Based Therapies: Scalable Stirred-Tank Bioreactor Mediated Manufacturing

doi: 10.3389/fmedt.2022.850565

Figure Lengend Snippet: The T cell activation in STR with ATF mediated continuous perfusion results in T cells with stemness over terminal differentiation or exhaustion: (A) Prominent subsets of T cells before, during and after expansion; (B) Differentiation status of T cells before, during and after expansion; and (C) Senescence and exhaustion of T cells before, during and after expansion. The values represent average of 50,000 single cells per condition.

Article Snippet: As the pore size of the hollow fibers in Repligen's ATF is 0.2 mm, we have employed ATF as a cell retention device for continuous media perfusion in STR.

Techniques: Activation Assay

Representation of closed, GMP compatible end-to-end platform for activation, expansion, selection and downstream processing of T cells. T cell activation is performed in the STR. Cell retention and continuous media perfusion is enabled by ATF. Depletion of undesired cells is performed in the STR with proprietary magnetic technology. Cells are concentrated and formulated using automated closed systems.

Journal: Frontiers in Medical Technology

Article Title: Enabling Allogeneic T Cell-Based Therapies: Scalable Stirred-Tank Bioreactor Mediated Manufacturing

doi: 10.3389/fmedt.2022.850565

Figure Lengend Snippet: Representation of closed, GMP compatible end-to-end platform for activation, expansion, selection and downstream processing of T cells. T cell activation is performed in the STR. Cell retention and continuous media perfusion is enabled by ATF. Depletion of undesired cells is performed in the STR with proprietary magnetic technology. Cells are concentrated and formulated using automated closed systems.

Article Snippet: As the pore size of the hollow fibers in Repligen's ATF is 0.2 mm, we have employed ATF as a cell retention device for continuous media perfusion in STR.

Techniques: Activation Assay, Selection